Neurofilament (NEFM) Rabbit Polyclonal Antibody

CAT#: TA336603

Rabbit Polyclonal NF-M Antibody

Product Images

Western Blot: 160kDa Neurofilament Medium Antibody TA336603 - Western blot analysis of 160kDa Neurofilament Medium expression on whole rat cerebellum homogenate using TA336603.

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  Immunocytochemistry/Immunofluorescence: 160kDa Neurofilament Medium Antibody TA336603 - Section of rat cerebral cortex stained with TA336603 (red), which reveals the perikarya of pyramidal neurons and dendrites and axons surrounding them. The green chan

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:5000-1:10000, IF: 1:500-1:1000, IHC: 1:1000-1:5000, IHC-F: 1:1000-1:5000, IHC-P: 1:1000-1:5000
• Reactivities: Bovine, Feline, Human, Mouse, Porcine, Rat, Avian
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant rat Neurofilament Medium fusion protein corresponding to the C-terminus [UniProt# P12839]
• Formulation: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Whole antisera
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 160 kDa
• Gene Name: neurofilament, medium polypeptide
• Database Link:
  NP_005373
  Entrez Gene 18040 MouseEntrez Gene 24588 RatEntrez Gene 4741 Human
  UniProt ID P07197
• Background: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called Neurofilament Light (NF-L), Neurofilament Medium (NF-M) and Neurofilament Heavy (NF-H). Neurofilament Medium is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170kDa, with some variation in different species. Antibodies to Neurofilament Medium are useful to identify this protein and identify neurons and their processes in tissue sections and in tissue culture. Neurofilament Medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
• Synonyms: NEF3; NF-M; NFM
• Note: This 160kDa Neurofilament Medium Antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, and Immunohistochemistry-Frozen. In Western blot a band can be seen around 145kDa corresponding to rodent Neurofilament Medium. Human and bovine Neurofilament Medium run a little slower and can be seen at about 160kDa.

Reference Data

• Protein Pathways: Amyotrophic lateral sclerosis (ALS)