c Maf (MAF) Rabbit Polyclonal Antibody

CAT#: TA337003

Rabbit Polyclonal c-Maf Antibody

Product Images

Western Blot: c-Maf Antibody TA337003 - Analysis of Lysate from human brain in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain probed at 3 ug/ml.

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  Western Blot: c-Maf Antibody TA337003 - Total protein from Human Pancreas and Human Small Intestine was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-cMaf in 1% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

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  Immunohistochemistry-Paraffin: c-Maf Antibody TA337003 - Staining of Human kidney probed with c-maf antibody at 10 ug/ml.

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  Immunohistochemistry-Paraffin: c-Maf Antibody TA337003 - Staining of Human placenta probed with c-maf antibody at 10 ug/ml.

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  Immunohistochemistry-Paraffin: c-Maf Antibody TA337003 - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human liver cancer using c-MAF antibody at 2ug/ml concentration (1:500 dilution). The primary antibody binding to c-MAF protein was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This c-MAF antibody showed a very strong nuclear and moderate to strong staining in the endothelial cells (blood vessels) and in a subset of apparently infiltrating inflammatory cells. Weak to moderate cytoplasmic and nuclear staining was observed in liver cancer cells and the cells of tumor stroma including fibroblasts.

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry-Paraffin: 1-2 ug/ml, Western Blot: 2-4 ug/ml, Immunohistochemistry
• Reactivities: Human, Mouse, Rat, Bovine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to amino acids 75-110 of human c-maf was used as the immunogen, GenBank no. NP_001026974.1|.
• Formulation: PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: MAF bZIP transcription factor
• Database Link:
  NP_001026974
  Entrez Gene 17132 MouseEntrez Gene 54267 RatEntrez Gene 4094 Human
  UniProt ID O75444
• Synonyms: c-MAF; CCA4; CTRCT21

Reference Data

• Protein Families: Druggable Genome, Transcription Factors