TLR9 Mouse Monoclonal Antibody [Clone ID: 26C593.2]
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Specifications
Product Data | |
Clone Name | 26C593.2 |
Applications | FC, IHC, WB |
Recommended Dilution | Immunohistochemistry, Block/Neutralize, Knockdown Validated, Functional, In vitro assay, ELISA: 1:100 - 1:2000, Immunoprecipitation: 1:10 - 1:500, Flow (Intracellular): 1:10 - 1:1000, Dot Blot, Western Blot: 2-5 ug/ml, Immunohistochemistry-Paraffin: 5 ug/ml, Flow Cytometry, Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Simple Western: 30 ug/ml, CyTOF-ready |
Reactivities | Human, Mouse, Rat, Canine, Equine, Primate |
Host | Mouse |
Isotype | IgG1, kappa |
Clonality | Monoclonal |
Immunogen | This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-300 of human TLR9 isoform A (Genbank accession no. AAF78037). |
Formulation | PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Concentration | lot specific |
Purification | Protein G purified |
Conjugation | Unconjugated |
Storage | Store at -20°C as received. |
Stability | Stable for 12 months from date of receipt. |
Gene Name | toll like receptor 9 |
Database Link | |
Synonyms | CD289 |
Note | Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. In human PBMC, a ~120 kDa band is observed. A smaller isoform, TLR9 isoform B (Genbank accession no. AAF72190) containing 975 amino acids may also be observed in some cases. |
Reference Data | |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Toll-like receptor signaling pathway |
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