GFI1 Rabbit Polyclonal Antibody

CAT#: TA337145

Rabbit Polyclonal GFI-1 Antibody

Product Images

Western Blot: GFI-1 Antibody TA337145 - Analysis of Zinc finger protein Gfi-1 in human HepG2 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) mouse RAW cell lysate using this antibody. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

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  Immunohistochemistry-Paraffin: GFI-1 Antibody TA337145 - Analysis of Gfi-1 in formalin-fixed, paraffin-embedded human brain tissue using this antibody at 5 ug/ml.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry, Western Blot: 6-8ug/ml~, Immunohistochemistry-Paraffin: 5ug/ml
• Reactivities: Human, Mouse, Rat, Bovine, Chicken
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A portion of amino acid 280-330 of human Zinc finger protein Gfi-1 was used as the immunogen.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein A purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: growth factor independent 1 transcriptional repressor
• Database Link:
  NP_005254
  Entrez Gene 14581 MouseEntrez Gene 24388 RatEntrez Gene 2672 Human
  UniProt ID Q99684
• Background: Gfi-1 is a nuclear zinc finger (ZF) transcriptional repressor that plays an important role in hematopoiesis and inner ear development, and has been implicated in lymphomagenesis. The Gfi1 protein expression is regulated by the ubiquitin-proteasome system. It is reported that ubiquitin ligase Triad1 interacts with the DNA-binding domain of Gfi-1. Gfi-1 represses transcription by directly binding to the consensus DNA sequence in the promoters of its target genes. Deficiency of Gfi-1 causes neutropenia and an accumulation of granulomonocytic precursor cells that is reminiscent of a myelodysplastic syndrome in mice.
• Synonyms: GFI-1; GFI1A; SCN2; ZNF163

Reference Data

• Protein Families: Druggable Genome, Transcription Factors