LIF Rat Monoclonal Antibody [Clone ID: 39N7D10]

CAT#: TA337172

Rat Monoclonal LIF Antibody (39N7D10)

Product Images

Western Blot: LIF Antibody (39N7D10) TA337172 - WB validation of LIF antibody (clone 39N7D10) on (A) full-length recombinant Lif protein, (B) mouse spleen lysate and (C) human spleen lysate. 3 ug/mL concentration of primary antibody, Goat anti-rat IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this assay.

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  Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) TA337172 - Tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10).

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  Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) TA337172 - Tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic immunepositivity of LIF was observed in the cuboidal epithelial cells of renal tubules.

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  Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) TA337172 - Tissue section of mouse kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Very intense immune positivity of LIF was observed in membranes as well as the cytoplasm of cuboidal epithelial cells of renal tubules.

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  Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) TA337172 - Tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as the goblet cells in the rectal glands depicted membrane-cytoplasmic immunostaining of LIF protein.

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  Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) TA337172 - Tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts developed intense membrane-cytoplasmic LIF immunostaining. Additionally, some cells in the lamina propria and the sub-mucosal layer also depicted weak positivity for LIF staining.

• Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) TA337172 - Tissue section of normal human prostate using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Cell surface/membrane- cytoplasmic immunepositivity of LIF was observed specifically in the epithelial cells of prostate alveolar glands, whereas the surrounding fibromuscular stroma cells did not develop any staining.

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Specifications

Product Data

• Clone Name: 39N7D10
• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry, Immunohistochemistry-Paraffin: 5 ug/mL, Western Blot: 3-5ug/ml~, Immunocytochemistry/ Immunofluorescence
• Reactivities: Human, Mouse
• Host: Rat
• Isotype: IgG2b, kappa
• Clonality: Monoclonal
• Immunogen: A recombinant murine Lif protein containing amino acids 24-203 was used as the immunogen for this antibody.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: leukemia inhibitory factor
• Database Link:
  NP_002300
  Entrez Gene 16878 MouseEntrez Gene 3976 Human
  UniProt ID P15018
• Background: Lif has long been recognized as a cytokine which promotes murine germ cell growth in the absence of feeder cell layers in mouse cells (1-3)Characterized initially as an inhibitor of differentiation in M1 murine monocytic leukemia lines (hence the name Leukemia Inhibitory Factor),Lif is now recognized as a key factor for maintaining stem cells in the undifferentiated state- at least in mouse embryonic stem cells (4)Acting through the Lif receptor and the induced activation of STAT3, murine ESCs can be maintained in vitro in undifferentiated stateHowever, human ESCs, although functionally stimulated with Lif, are not similarly maintained as undifferentiatedThis illustrates a different functional program for Lif in mice and humans in maintenance of stem cellsAn IL-6 family member, Lif is an important pleiotropic cytokine with activity on cells of hematopoietic and non-hematopoietic origin.
• Synonyms: CDF; DIA; HILDA; MLPLI
• Note: The Lif protein can be highly glycosylated and has been observed between 22 kD and 34 kD in western blotting. In IHC-P application, 1M EDTA buffer pH 9.0 was used for antigen retrieval (citrate buffer pH 6.0 did not work for this target).

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway