LIF Rat Monoclonal Antibody [Clone ID: 39N7D10]

CAT#: TA337172

Rat Monoclonal LIF Antibody (39N7D10)


USD 424.00

5 Days*

Size
    • 100 ug

Frequently bought together (3)
Recombinant protein of human leukemia inhibitory factor (cholinergic differentiation factor) (LIF)
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beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
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Specifications

Product Data
Clone Name 39N7D10
Applications IHC, WB
Recommended Dilution Immunohistochemistry, Immunohistochemistry-Paraffin: 5 ug/mL, Western Blot: 3-5ug/ml~, Immunocytochemistry/ Immunofluorescence
Reactivities Human, Mouse
Host Rat
Isotype IgG2b, kappa
Clonality Monoclonal
Immunogen A recombinant murine Lif protein containing amino acids 24-203 was used as the immunogen for this antibody.
Formulation PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration lot specific
Purification Protein G purified
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Gene Name leukemia inhibitory factor
Background Lif has long been recognized as a cytokine which promotes murine germ cell growth in the absence of feeder cell layers in mouse cells (1-3)Characterized initially as an inhibitor of differentiation in M1 murine monocytic leukemia lines (hence the name Leukemia Inhibitory Factor),Lif is now recognized as a key factor for maintaining stem cells in the undifferentiated state- at least in mouse embryonic stem cells (4)Acting through the Lif receptor and the induced activation of STAT3, murine ESCs can be maintained in vitro in undifferentiated stateHowever, human ESCs, although functionally stimulated with Lif, are not similarly maintained as undifferentiatedThis illustrates a different functional program for Lif in mice and humans in maintenance of stem cellsAn IL-6 family member, Lif is an important pleiotropic cytokine with activity on cells of hematopoietic and non-hematopoietic origin.
Synonyms CDF; DIA; HILDA; MLPLI
Note The Lif protein can be highly glycosylated and has been observed between 22 kD and 34 kD in western blotting. In IHC-P application, 1M EDTA buffer pH 9.0 was used for antigen retrieval (citrate buffer pH 6.0 did not work for this target).
Reference Data
Protein Families Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.