CSK Mouse Monoclonal Antibody [Clone ID: 2B4-C6-A10]

CAT#: TA346925

Mouse Monoclonal CSK Antibody

Product Images

Western blot detection of CSK in C6 and Jurkat cell lysates using CSK mouse mAb (1:1000 diluted).Predicted band size:50KDa.Observed band size:50KDa.

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  Immunocytochemistry staining of C6 cells fixed by anhydrous methanol for 2 h at -20? and using anti-CSK mouse mAb (dilution 1:50).

Specifications

Product Data

• Clone Name: 2B4-C6-A10
• Applications: IF, WB
• Recommended Dilution: WB: 1:1000, IF: 1:50
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: The immunogen for CSK antibody: purified recombinant human CSK protein fragments expressed in E.coli.
• Formulation: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol.
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50 kDa
• Gene Name: c-src tyrosine kinase
• Database Link:
  NP_001120662
  Entrez Gene 315707 RatEntrez Gene 1445 Human
  UniProt ID P41240
• Background: Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.
• Synonyms: MGC117393

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, Neurotrophin signaling pathway, Regulation of actin cytoskeleton