ATG4B Rabbit Polyclonal Antibody

CAT#: TA349132

Rabbit Polyclonal Anti-ATG4B Antibody

Product Images

Western blot analysis of ATG4B in 3T3 cell lysate with ATG4B antibody at 1 ug/mL.

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  Immunohistochemistry of ATG4B in human spleen tissue with ATG4B antibody at 5 ug/mL.

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  Immunofluorescence of ATG4B in human spleen tissue with ATG4B antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, IHC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: ATG4B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ATG4B.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: ATG4B antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 43 kDa; Observed: 44 kDa
• Gene Name: autophagy related 4B cysteine peptidase
• Database Link:
  NP_037457
  Entrez Gene 66615 MouseEntrez Gene 316640 RatEntrez Gene 23192 Human
  UniProt ID Q9Y4P1
• Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4B, also known as AUTL1, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3). ATG4 is required for ATG8 conjugation to phosphatidylethanolamine on autophagosomal membranes. In mammals, each ATG4 homolog shows a selective preference for the ATG8 homologs (4). ATG4B has been found to be a novel protective protein in inflammatory colitis (5).
• Synonyms: APG4B; AUTL1

Reference Data

• Protein Families: Protease
• Protein Pathways: Regulation of autophagy