SPARC Rabbit Polyclonal Antibody

CAT#: TA349441

Rabbit Polyclonal Anti-SPARC rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane: A375 cells Primary antibody: TA349441 (SPARC Antibody) at dilution 1/500 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds

• 

  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA349441 (SPARC Antibody) at dilution 1/35 (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA349441 (SPARC Antibody) at dilution 1/35, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: A375 cells
  IHC: 25-100
  Positive control: Human liver cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human SPARC
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35 kDa
• Gene Name: secreted protein acidic and cysteine rich
• Database Link:
  NP_003109
  Entrez Gene 20692 MouseEntrez Gene 24791 RatEntrez Gene 6678 Human
  UniProt ID P09486
• Background: This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion.
• Synonyms: BM-40; OI17; ON

Reference Data

• Protein Families: Druggable Genome