IDH2 Rabbit Polyclonal Antibody

CAT#: TA350076

Rabbit Polyclonal Anti-IDH2 Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-3: 293T cells Jurkat cells human fetal muscle tissue Primary antibody: TA350076 (IDH2 Antibody) at dilution 1/600 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds

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  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA350076 (IDH2 Antibody) at dilution 1/60 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA350076 (IDH2 Antibody) at dilution 1/60, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA350076 (IDH2 Antibody) at dilution 1/60 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA350076 (IDH2 Antibody) at dilution 1/60, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: 293T and Jurkat cells, human fetal muscle tissue
  IHC: 100-300
  Positive control: Human cervical cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human IDH2
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51 kDa
• Gene Name: isocitrate dehydrogenase (NADP(+)) 2, mitochondrial
• Database Link:
  NP_002159
  Entrez Gene 269951 MouseEntrez Gene 361596 RatEntrez Gene 3418 Human
  UniProt ID P48735
• Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.
• Synonyms: D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH

Reference Data

• Protein Pathways: Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways