POLR2A Mouse Monoclonal Antibody [Clone ID: CTD 4H8]

CAT#: TA353393L

Mouse monoclonal Anti-RNA polII Clone CTD 4H8

Specifications

Product Data

• Clone Name: CTD 4H8
• Recommended Dilution: ChIP ; ELISA ; FACS ; IHC ; IF ; IP ; WB
• Reactivities: Human, Saccharomyces cerevisiae
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Peptide: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
• Concentration: lot specific
• Purification: Purified by affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: polymerase (RNA) II subunit A
• Database Link:
  NP_000928
  Entrez Gene 5430 Human
  UniProt ID P24928
• Background: RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII. Mutations that directly affect transcription by RNA polymerases rank among the most central mediators of malignant transformation. CTD4H8 can also be used in chromatin immunoprecipitation (ChIP) assays.
• Synonyms: hRPB220; hsRPB1; POLR2; POLRA; RPB1; RPBh1; RpIILS; RPO2; RPOL2
• Note: Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein.

Reference Data

• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase