CDK8 Rabbit Polyclonal Antibody

CAT#: TA354382

Rabbit anti Cdk8 Polyclonal Antibody

Product Images

WB: The cell lysate derived from HT29 was resolved on to 10% SDSPAGE, transferred to NC membrane and immunoprobed by the Rabbit anti-Cdk8 antibody, at 1:500 for 30 min at RT. An immunereactive band is observed around 54 kDa.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide derived from C-terminus of human cdk8 protein. This sequence is identical to human, mouse and rat.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54 kDa
• Gene Name: cyclin-dependent kinase 8
• Database Link:
  NP_001251
  Entrez Gene 264064 MouseEntrez Gene 498140 RatEntrez Gene 1024 Human
  UniProt ID P49336
• Background: A family of proteins designated as cdks belongs to the protein kinase superfamily. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified. CDK8 kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.
• Synonyms: K35

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors