COX4 (COX4I1) Rabbit Polyclonal Antibody

CAT#: TA356212

COX4I1 Antibody - N-terminal region

Product Images

WB Suggested Anti-COX4I1 Antibody Titration: 1.25ug/ml Positive Control: HepG2 cell lysateCOX4I1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells

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  COX4I1 antibody - N-terminal region (TA356212) validated by WB using 1. Human liver 2. Rat liver 3. Wild-type mouse liver 4. AMPKa1+2-/- mouse liver 5. Human muscle 6. Rat muscle 7. Mouse muscle at 1:1000.

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  Lanes: Lane 1: 50ug HeLa lysate Lane 2: 50ug 293T lysate Lane 3: 50ug K562 lysate Lane 4: 50ug MDA-MB-231 lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1:1000 Gene Name: COX4I1 Submitted by: David Colecchia, Ph.D, Istituto Toscano Tumori, Core Research Laboratory, presso Fondazione Toscana Life Sciences

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  Human Heart

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  Human kidney

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  Rabbit Anti-COX4I1 Antibody Catalog Number: ARP42784 Paraffin Embedded Tissue: Human cardiac cell Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X

• COX4I1 was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with TA356212 with 1:200 dilution. Western blot was performed using TA356212 at 1/1000 dilution. Lane 1: Control IP in HEK293 Whole Cell Lysate. Lane 2: COX4I1 IP with TA356212 in HEK293 Whole Cell Lysate. Lane 3: Input of HEK293 Whole Cell Lysate.

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Specifications

Product Data

• Applications: IHC, IP, WB
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human COX4I1
• Specificity: Expected reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
  Homology: Cow: 86%; Dog: 86%; Guinea Pig: 86%; Horse: 86%; Human: 100%; Mouse: 85%; Rabbit: 86%; Rat: 86%
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: lot specific
• Purification: Protein A purified
• Conjugation: Unconjugated
• Storage: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Predicted Protein Size: 19kDa
• Gene Name: cytochrome c oxidase subunit 4I1
• Database Link:
  NP_001852
  Entrez Gene 1327 Human
  UniProt ID P13073
• Background: Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. COX4I1 is the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme.Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it.
• Synonyms: COX4; COXIV; MGC72016

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease