Dynamin 1 (DNM1) Rabbit Polyclonal Antibody

CAT#: TA356977

DNM1 Antibody - middle region

Product Images

WB Suggested Anti-DNM1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:12500 Positive Control: Human brain

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  Sample Type: 3. rat brain extract (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical ScienceÂ

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  Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical Science Application: IHC Species+tissue/cell type: Mouse brain stem cells Primary antibody dilution: 1:500 Secondary antibody: Goat anti-rabbit Alexa-Fluor 594 Secondary antibody dilution: 1:1000

Specifications

Product Data

• Applications: IHC, WB
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DNM1
• Specificity: Expected reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat, Zebrafish
  Homology: Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 86%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 86%
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Predicted Protein Size: 95kDa
• Gene Name: dynamin 1
• Database Link:
  NP_004399
  Entrez Gene 13429 MouseEntrez Gene 140694 RatEntrez Gene 1759 Human
  UniProt ID P39053
• Background: DNM1 is a member of the dynamin subfamily of GTP-binding proteins. The protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described.This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described.
• Synonyms: DNM

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis