Actin (ACTA1) Rabbit Polyclonal Antibody

CAT#: TA357777

ACTA1 Antibody - C-terminal region

Product Images

WB Suggested Anti-ACTA1 Antibody Titration: 1.0 ug/ml Positive Control: A549 Whole Cell

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  WB Suggested Anti-ACTA1 Antibody Positive Control: Lane 1: 50ug term baboon muscle homogenate Primary Antibody Dilution : 1:1666 Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:1200 Submitted by: Cynthia Blanco, University of Texas Health Science Center

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  Rabbit Anti-ACTA1 Antibody Catalog Number: TA357777 Formalin Fixed Paraffin Embedded Tissue: Human Heart Muscle Tissue Observed Staining: Cytoplasm Primary Antibody Concentration: 1:100 Other Working Concentrations: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec

Specifications

Product Data

• Applications: WB
• Reactivities: Human, Baboon
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: Expected reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
  Homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 100%; Zebrafish: 100%
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Predicted Protein Size: 42kDa
• Gene Name: actin, alpha 1, skeletal muscle
• Database Link:
  NP_001091
  Entrez Gene 58 Human
  UniProt ID P68133
• Background: The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
• Synonyms: ACTA; Alpha-actin-1; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3

Reference Data

• Protein Families: Stem cell - Pluripotency