SPARC Rabbit Polyclonal Antibody

CAT#: TA358367

SPARC Antibody - C-terminal region

Product Images

WB Suggested Anti-SPARC Antibody Titration: 1.0 ug/ml Positive Control: PANC1 Whole CellSPARC is strongly supported by BioGPS gene expression data to be expressed in Human PANC1 cells

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  Sample Type: Adult mouse tectum Primary Antibody Dilution: 1:500 Secondary Antibody: Anti-rabbit-Cy3 Secondary Antibody Dilution: 1:1000 Color/Signal Descriptions: Red: Sparc Cyan: Nissl(Neurons) Gene Name: SPARC Submitted by: Joshua R. Sanes, Molecular and Cellular Biology, Harvard University, 52 Oxford Street, Room 335, Cambridge MA 02138, Phone: 617-496-8683, FAX: 617-495-0524, email: sanesj@mcb.harvard.edu

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  Rabbit Anti-SPARC Antibody Catalog Number: TA358367 Formalin Fixed Paraffin Embedded Tissue: Human Testis Tissue Observed Staining: Plasma membrane and cytoplasm in spermatogonia and spermatocytes Primary Antibody Concentration: 1:100 Other Working Concentrations: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec

Specifications

Product Data

• Applications: IHC, WB
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: Expected reactivity: Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
  Homology: Cow: 93%; Dog: 86%; Goat: 93%; Guinea Pig: 86%; Horse: 86%; Human: 100%; Mouse: 79%; Rabbit: 93%; Rat: 92%; Sheep: 93%; Zebrafish: 77%
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Predicted Protein Size: 35kDa
• Gene Name: secreted protein acidic and cysteine rich
• Database Link:
  NP_003109
  Entrez Gene 20692 MouseEntrez Gene 6678 Human
  UniProt ID P09486
• Background: Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM).
• Synonyms: BM-40; ON; Osteonectin

Reference Data

• Protein Families: Druggable Genome