Corticotropin Releasing Factor (CRH) Rabbit Polyclonal Antibody

CAT#: TA363902

Corticotropin-Releasing Factor (CRF), rabbit anti human/mouse/rat, polyclonal, diluted Antiserum for RIA.

Product Images

Typical titration curve of Somatostatin-14 in a competitive ELISA with this antibody

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: This antibody has been tested and validated in ELISA against Corticotropin Releasing Factor. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide H-Ser-Glu-Glu-Pro-Pro-Ile-Ser-Leu-Asp-Leu-Thr- Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Ala-Arg-Ala-Glu-Gln- Leu-Ala-Gln-Gln-Ala-His-Ser-Asn-Arg-Lys-Leu-Met-Glu-Ile-Ile-NH2 coupled to carrier protein.
• Formulation: This polyclonal antibody is supplied as a lyophilized powder. The powder should be rehydrated with 50ml of RIA buffer. Upon reconstitution to 50ml total volume, the solution contains 0.1M sodium phosphate buffer (pH 7.4), 0.05M NaCl, 0.1% BSA, 0.01% NaN3, and 0.1% Triton X-100. Store at 4° - 8°C. This should ensure antibody stability for approximately one month.
• Concentration: N/A
• Conjugation: Unconjugated
• Storage: Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
• Gene Name: corticotropin releasing hormone
• Database Link:
  Entrez Gene 1392 Human
  UniProt ID P06850
• Background: Corticotropin-Releasing Factor (CRF, also known as Corticoliberin) is a 41 amino acid peptide hormone derived from the 196-amino acid preprohormone. It is secreted in the hypothalamus in response to stress, and stimulates both the synthesis and the secretion of adrenocorticotropic hormone (ACTH). CRF is also synthesized in peripheral tissues and placenta and it is known to suppress appetite and increase subjective feelings of anxiety. This antibody was generated by immunization of rabbits with CRF coupled to a carrier protein.
• Synonyms: CRF