PENK Rabbit Polyclonal Antibody

CAT#: TA364196

Rabbit polyclonal anti Peptide F (bo); purified rabbit IgG

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: This antibody has been tested and validated in ELISA against Peptide F. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
• Reactivities: Bovine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide H-Tyr-Gly-Gly-Phe-Met-Lys-Lys-Met-Asp-Glu-Leu-Tyr- Pro-Leu-Glu-Val-Glu-Glu-Glu-Ala-Asn-Gly-Gly-Glu-Val-Leu-Gly-Lys-Arg- Tyr-Gly-Gly-Phe-Met-OH coupled to a carrier protein.
• Formulation: Protein A affinity purified from antiserum, lyophilized, packaged under nitrogen. Reconstitute by adding 0.2ml distilled water. This stock solution contains 2mg/ml IgG, phosphate buffer saline pH 7.4 (PBS), and 0.02% (w/v) Thimerosal as a preservative.
• Concentration: N/A
• Conjugation: Unconjugated
• Storage: Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
• Database Link:
  Entrez Gene 281387 Bovine
  UniProt ID P01211
• Background: Proenkephalin, also known as proenkephalin A, is an endogenous opioid polypeptide hormone which, via proteolyic cleavage, produces the enkephalin peptides [Met]enkephalin, and to a lesser extent, [Leu]enkephalin. The pre-proenkephalin gene contains four copies of Met-enkephalin and one copy each of Leu-enkephalin, the heptapeptide Met-enkephalin-Arg- Phe, and the octapeptide Met-Enkephalin-Arg-Gly-Leu. Other endogenous opioid peptides produced by proenkephalin include adrenorphin, amidorphin, BAM-18, BAM-20P, BAM-22P, peptide B, peptide E, and peptide F. Peptide F contains [Met5]enkephalin sequences at both its N terminus and its C terminus. The carboxy-terminally amidated peptide comprising the first 26 amino acids of peptide F is named amidorphin. This antibody was generated by immunization of rabbits with Peptide F coupled to a carrier protein.
• Synonyms: preproenkephalin; proenkephalin