Hamp Rabbit Polyclonal Antibody

CAT#: TA364352

Rabbit polyclonal anti Hepcidin-25 (ms); purified rabbit IgG

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: This antibody has been tested and validated in ELISA against Hepcidin- 25. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide H-Asp-Thr-Asn-Phe-Pro-Ile-Cys-Leu-Phe-Cys-Cys- Lys-Cys-Cys-Lys-Asn-Ser-Ser-Cys-Gly-Leu-Cys-Cys-Ile-Thr-OH coupled to carrier protein.
• Formulation: Protein A affinity purified from antiserum, lyophilized, packaged under nitrogen. Reconstitute by adding 0.2ml distilled water. This stock solution contains 2mg/ml IgG, phosphate buffer saline pH 7.4 (PBS), and 0.02% (w/v) Thimerosal as a preservative.
• Concentration: N/A
• Conjugation: Unconjugated
• Storage: Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
• Gene Name: hepcidin antimicrobial peptide
• Database Link:
  Entrez Gene 84506 Mouse
  UniProt ID Q9EQ21
• Background: Hepcidin-25 is a 25-residue peptide synthesized in the liver. This is the bioactive form of hepcidin, a key regulator of iron homeostasis. It acts through downregulation of the cellular iron exporter ferroportin, which is expressed on enterocytes, macrophages and hepatocytes. Hepcidin-25 production is upregulated by inflammation as well as iron overload, leading to anemia through interference with the body's intestinal iron absorption and its utilization of iron. Hepcidin is also a small defensin-like peptide with anti-microbial activity, which is secreted into the urine. This antibody was generated by immunization of rabbits with Hepcidin-25 coupled to a carrier protein.
• Synonyms: HEPC; HEPCIDIN; HFE2; HFE2B; LEAP-1; LEAP1; PLTR