CDK4 Rabbit Polyclonal Antibody

CAT#: TA364436

CDK4 rabbit polyclonal antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 20 μg Lane: Hela cell lysate Primary antibody: TA364436 (CDK4 Antibody) at dilution 1/1000 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 90 seconds

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  Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA364436 (CDK4 Antibody) at dilution 1/60 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA364436 (CDK4 Antibody) at dilution 1/60, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Hela cell lysate
  IHC: 50-300
  Positive control: Human esophagus cancer
  Predicted cell location: Nucleus and Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human CDK4
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 34 kDa
• Database Link:
  UniProt ID P11802
• Background: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.