PRAS40 (AKT1S1) Rabbit Polyclonal Antibody

CAT#: TA373422

Phospho-AKT1S1/PRAS40-T246 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of HeLa cells, using Phospho-AKT1S1/PRAS40-T246 antibody (TA373422) at 1:2000 dilution. HeLa cells were treated by Insulin (0.01U/mL) for 15 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 90s.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: WB,1:500 - 1:2000
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human
• Modifications: Phospho T246
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 27kDa
• Gene Name: AKT1 substrate 1
• Database Link:
  Entrez Gene 84335 Human
  UniProt ID Q96B36
• Background: AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).
• Synonyms: Lobe; MGC2865; OTTHUMP00000196759; OTTHUMP00000196760; PRAS40