15 Lipoxygenase 2 (ALOX15B) Rabbit Polyclonal Antibody

CAT#: TA373468

ALOX15B Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using ALOX15B antibody (TA373468) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 2s.

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  Immunofluorescence analysis of NIH/3T3 cells using ALOX15B Rabbit pAb (TA373468) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of PC-12 cells using ALOX15B Rabbit pAb (TA373468) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U2OS cells using ALOX15B Rabbit pAb (TA373468) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ELISA, ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:1000
  IF/ICC,1:50 - 1:200
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 76kDa
• Gene Name: arachidonate 15-lipoxygenase, type B
• Database Link:
  Entrez Gene 247 Human
  UniProt ID O15296
• Background: This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described.
• Synonyms: 15-LOX-2; 15S-lipoxygenase