CDK8 Rabbit Polyclonal Antibody

CAT#: TA374574

CDK8 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts from normal (control) and CDK8 knockout (KO) HeLa cells, using CDK8 antibody (TA374574) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Western blot analysis of extracts of Jurkat cells, using CDK8 antibody (TA374574) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

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  Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug CDK8 antibody (TA374574). Western blot was performed from the immunoprecipitate using CDK8 antibody (TA374574) at a dilition of 1:1000.

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 53kDa
• Gene Name: cyclin-dependent kinase 8
• Database Link:
  Entrez Gene 1024 Human
  UniProt ID P49336
• Background: This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.
• Synonyms: K35; MGC126074; MGC126075