PFKFB2 Rabbit Polyclonal Antibody

CAT#: TA379830

Phospho-PFKFB2-S483 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of HeLa cells, using Phospho-PFKFB2-S483 antibody (TA379830) at 1:2000 dilution.HeLa cells were treated by IGF-1 (50 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 5s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human
• Modifications: Phospho S483
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around S483 of human PFKFB2 (NP_006203.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 54kDa/58kDa
• Gene Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
• Database Link:
  Entrez Gene 5208 Human
  UniProt ID O60825
• Background: The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene.
• Synonyms: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatas; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphata; DKFZp781D2217; MGC138308; MGC138310; PFK-2/FBPase-2