POLA2 Rabbit Polyclonal Antibody

CAT#: TA380118

POLA2 Rabbit polyclonal Antibody

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:1000 - 1:3000
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human POLA2 (NP_002680.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 40kDa/65kDa
• Gene Name: DNA polymerase alpha 2, accessory subunit
• Database Link:
  Entrez Gene 23649 Human
  UniProt ID Q14181
• Background: Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex which plays an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2 is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1 (By similarity. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands (By similarity. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity.