TRPV6 Rabbit Polyclonal Antibody

CAT#: TA382879

TRPV6 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using TRPV6 antibody (TA382879) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

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  Immunohistochemistry of paraffin-embedded rat stomach using TRPV6 Rabbit pAb (TA382879) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TRPV6 (NP_061116.5).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 68kDa/87kDa
• Gene Name: transient receptor potential cation channel subfamily V member 6
• Database Link:
  Entrez Gene 55503 Human
  UniProt ID Q9H1D0
• Background: This gene encodes a member of a family of multipass membrane proteins that functions as calcium channels. The encoded protein contains N-terminal ankyrin repeats, which are required for channel assembly and regulation. Translation initiation for this protein occurs at a non-AUG start codon that is decoded as methionine. This gene is situated next to a closely related gene for transient receptor potential cation channel subfamily V member 5 (TRPV5). This locus has experienced positive selection in non-African populations, resulting in several non-synonymous codon differences among individuals of different genetic backgrounds.
• Synonyms: ABP/ZF; CaT-L; CaT-like; CaT1; CATL; ECAC2; HSA277909; LP6728; ZFAB