YAP1 Rabbit Polyclonal Antibody

CAT#: TA383392

YAP1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts from normal (control) and YAP1 Rabbit pAb knockout (KO) HeLa cells, using YAP1 Rabbit pAb antibody (TA383392) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Western blot analysis of extracts of various cell lines, using YAP1 antibody (TA383392) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Immunofluorescence analysis of HeLa cells using YAP1 Polyclonal Antibody (TA383392) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH-3T3 cells using YAP1 Polyclonal Antibody (TA383392) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of PC-12 cells using YAP1 Polyclonal Antibody (TA383392) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Confocal immunofluorescence analysis of U2OS cells using YAP1 Polyclonal Antibody (TA383392) at dilution of 1:200. Blue: DAPI for nuclear staining.

• Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug YAP1 antibody (TA383392). Western blot was performed from the immunoprecipitate using YAP1 antibody (TA383392) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 36kDa/48kDa/49kDa/50kDa/52kDa/53kDa/54kDa
• Gene Name: Yes associated protein 1
• Database Link:
  Entrez Gene 10413 Human
  UniProt ID P46937
• Background: This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Synonyms: YAP; YAP2; YAP65; YKI