GAS41 (YEATS4) Rabbit Polyclonal Antibody

CAT#: TA383407

YEATS4 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of 293T cells, using YEATS4 antibody (TA383407) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 180s.

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  Immunohistochemistry of paraffin-embedded rat lung using YEATS4 Rabbit pAb (TA383407) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded human colon carcinoma using YEATS4 Rabbit pAb (TA383407) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded mouse lung using YEATS4 Rabbit pAb (TA383407) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunofluorescence analysis of A-549 cells using YEATS4 Rabbit pAb (TA383407) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of HeLa cells using YEATS4 Rabbit pAb (TA383407) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

• Immunofluorescence analysis of U2OS cells using YEATS4 Rabbit pAb (TA383407) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-227 of human YEATS4 (NP_006521.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 26kDa
• Gene Name: YEATS domain containing 4
• Database Link:
  Entrez Gene 8089 Human
  UniProt ID O95619
• Background: The protein encoded by this gene is found in the nucleoli. It has high sequence homology to human MLLT1, and yeast and human MLLT3 proteins. Both MLLT1 and MLLT3 proteins belong to a class of transcription factors, indicating that the encoded protein might also represent a transcription factor. This protein is thought to be required for RNA transcription. This gene has been shown to be amplified in tumors. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Synonyms: 4930573H17Rik; B230215M10Rik; GAS41; NUBI-1; NuBI1; YAF9