YES1 Rabbit Polyclonal Antibody

CAT#: TA383408S

YES1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using YES1 antibody (TA383408) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 20s.

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  Immunofluorescence analysis of U2OS cells using YES1 Rabbit pAb (TA383408) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:1000
  IF,1:50 - 1:100
• Reactivities: Human, Mouse
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human YES1 (NP_005424.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 60kDa
• Gene Name: YES proto-oncogene 1, Src family tyrosine kinase
• Database Link:
  Entrez Gene 7525 Human
  UniProt ID P07947
• Background: This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22.
• Synonyms: c-yes; HsT441; OTTHUMP00000162194; p61-Yes; Yes