PMS2 Rabbit Polyclonal Antibody

CAT#: TA384997

PMS2 Rabbit polyclonal Antibody

Product Images

Immunohistochemical analysis of paraffin-embedded human tonsil, using PMS2 Antibody.

• 

  Western blot analysis of PMS2 in (1) Jurkat cell lysate; (2)HeLa cell lysate.

Specifications

Product Data

• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/50-1/200
  ICC/IF: 1/50-1/200
  IP: 1/50
  FC: 1/50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthesized peptide derived from human PMS2
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 110kDa
• Gene Name: PMS1 homolog 2, mismatch repair system component
• Database Link:
  Entrez Gene 5395 Human
  UniProt ID P54278
• Background: Swiss-Prot Acc.P54278.Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected.
• Synonyms: HNPCC4; H_DJ0042M02.9; PMS2CL; PMSL2