POU2F1 Rabbit Monoclonal Antibody [Clone ID: R02-7K5]

CAT#: TA385013

N WASP Rabbit monoclonal Antibody

Product Images

Western blot detection of N-WASP in 3T3 cell lysates using N-WASP Rabbit mAb(1:1000 diluted).Predicted band size:55kDa.Observed band size:65kDa.

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  Immunohistochemistry of N-WASP in paraffin-embedded Human colon cancer tissue using N-WASP Rabbit mAb at dilution 1/20

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  Immunocytochemistry of N-WASP(green) in Hela cells using N-WASP Rabbit mAb at dilution 1/5, and DAPI(blue)

Specifications

Product Data

• Clone Name: R02-7K5
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/1000-1/2000
  IHC: 1/20-1/50
  ICC/IF: 1/50-1/100
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human N-WASP
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 55 kDa; Observed MW: 65 kDa
• Database Link:
  UniProt ID O00401
• Background: Swiss-Prot Acc.O00401.Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex (PubMed:9422512, PubMed:16767080, PubMed:19366662, PubMed:19487689, PubMed:22847007, PubMed:22921828). Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization (PubMed:9422512, PubMed:19366662, PubMed:19487689, PubMed:22847007, PubMed:22921828). Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia (PubMed:9422512). In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization (PubMed:16767080). Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression . Plays a role in dendrite spine morphogenesis .