alpha Tubulin (TUBA4A) Mouse Monoclonal Antibody [Clone ID: 4C9-6H6-4F7]

CAT#: TA385483

alpha Tubulin (4C9) Mouse monoclonal Antibody

Product Images

Immunohistochemical analysis of paraffin-embedded human Colon caricnoma using alpha tubulin mouse mAb diluted at 1:500.

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  Western blot detection of alpha Tubulin in MCF7,3T3,Rat Brain,Jurkat and Hela cell lysates using alpha tubulin mouse mAb (1:200000 diluted).Predicted band size:52kDa.Observed band size:52kDa.

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  IF analysis of Hela with (Left) and DAPI (Right) diluted at 1:100.

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  1) Input: Mouse Brain Tissue Lysate 2) IP product: IP dilute 1:2

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  Western blot detection of alpha Tubulin in A549,K562,THP-1,Rat Brain,Hela cell lysates using alpha Tubulin (4C9) Mouse mAb(1:5000 diluted).Predicted band size:52KDa.Observed band size:52KDa.

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  Western blot detection of alpha Tubulin (4C9) in Rat brain ,Jurkat,3T3 cell lysates using alpha Tubulin (4C9) antibody. Observed band size:52kDa.

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Specifications

Product Data

• Clone Name: 4C9-6H6-4F7
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/500-10000
  IP: 1/20
  ICC: 1/200
  IHC: 1/50-300
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Protein of Tubulin alpha-1A chain
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW (kDa):52
• Gene Name: tubulin alpha 4a
• Database Link:
  Entrez Gene 7277 Human
  UniProt ID P68363
• Background: Swiss-Prot Acc.P68363.Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.SitesFeature keyPosition(s)Description
• Synonyms: FLJ30169; H2-ALPHA; TUBA1