VPS4A Rabbit Monoclonal Antibody [Clone ID: R01-9H9]

CAT#: TA385554

VPS4A Rabbit monoclonal Antibody

Product Images

Western blot detection of VPS4a in K562,Rat Brain,C6,3T3 cell lysates using VPS4a Rabbit mAb(1:1000 diluted).Predicted band size:49kDa.Observed band size:49kDa.

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  Immunocytochemistry of VPS4a(green) in Hela cells using VPS4a Rabbit mAb at dilution 1/50, and DAPI(blue)

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  Immunohistochemistry of VPS4a in paraffin-embedded Human breast cancer tissue using VPS4a Rabbit mAb at dilution 1/5

Specifications

Product Data

• Clone Name: R01-9H9
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/2000-1/10000
  IHC: 1/500
  ICC/IF: 1/100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human VPS4a
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 49 kDa; Observed MW: 49 kDa
• Gene Name: vacuolar protein sorting 4 homolog A
• Database Link:
  Entrez Gene 27183 Human
  UniProt ID Q9UN37
• Background: Swiss-Prot Acc.Q9UN37.Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission (PubMed:24814515). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
• Synonyms: FLJ22197; hVPS4; SKD1; SKD1-homolog; SKD1A; SKD2; VPS4; VPS4-1