Annexin A1 (ANXA1) Rabbit Monoclonal Antibody [Clone ID: SAIC-13B-19]

CAT#: TA385885

Recombinant Anti-ANXA1 (Clone SAIC-13B-19)

Product Images

Flow cytometry using the Anti-ANXA1 antibody SAIC-13B-19 (Ab00306). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of SAIC-13B-19 (TA385885, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

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  Western Blot using anti-ANXA1 antibody SAIC-13B-19 (Ab00306). A431(A) (0.1µg/ml), A549(B) (0.1µg/ml), HeLa(C) (0.1µg/ml), and K562(D) (0.3µg/ml) cell lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-13B-19 (TA385885), before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Specifications

Product Data

• Clone Name: SAIC-13B-19
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Peptide "AAYLQETGKPLDETLK" derived from Annexin A1 conjugated to KLH.
• Specificity: Recognises human ANXA1.
  
  Original data characterizing this antibody may be found here .
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: annexin A1
• Database Link:
  Entrez Gene 301 Human
  UniProt ID P04083
• Synonyms: Annexin-1; ANX1; Chromobindin-9; LPC1; OTTHUMP00000021475; p35