Clusterin (CLU) Rabbit Monoclonal Antibody [Clone ID: SAIC-43B-8]

CAT#: TA385915

Recombinant Anti-CLU (Clone SAIC-43B-8)

Product Images

Flow cytometry using the Anti-CLU antibody SAIC-43B-8 (Ab00336). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of SAIC-43B-8 (TA385915, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

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  Western Blot using anti-CLU antibody SAIC-43B-8 (Ab00336). HeLa(A) (0.03µg/ml), MCF7(B) (0.01ug/ml) cell lysate and human brain cerebellum(C) (0.03ug/ml), human plasma (D) (0.03ug/ml) and human testes(E) (0.03ug/ml) tissue lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-43B-8 (TA385915) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Specifications

Product Data

• Clone Name: SAIC-43B-8
• Applications: ELISA, WB
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Peptide "ASSIIDELFQDR" derived from Clusterin conjugated to KLH. Clusterin isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins
• Specificity: Recognises human CLU.
  
  Original data characterizing this antibody may be found here .
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: clusterin
• Database Link:
  Entrez Gene 1191 Human
  UniProt ID P10909
• Synonyms: AAG4; Apo-J; APOJ; CLI; clusterin; KUB1; MGC24903; NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2