GABA A Receptor alpha 1 (GABRA1) Rabbit Monoclonal Antibody [Clone ID: 1F4]

CAT#: TA386322

Recombinant Anti-GABAR (Clone 1F4)

Specifications

Product Data

• Clone Name: 1F4
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was raised against α1β2γ2 GABA-A receptor.
• Specificity: This antibody recognises α1β2γ2 GABA-A receptor which is found in up to 50% of the synapses in the brain. It's ligand, GABA is a major inhibitory transmitter in the nervous system, binds to this receptor and opens a channel permeable to chloride. Disfunctions of GABAR are found in anxiety, neurodevelopmental disorderds and epilepsy.
  
  FSEC: High affinity and specificity of the 1F4 antibody to α1β2γ2 GABA-A receptor was demonstrated using fluorescence-detection size exclusion chromatography with GFP-tagged receptor (shift in elution volume) (Zhu et al., 2018). Crystallography: Furthermore, this antibody was utilised in production and purification of the α1β2γ2 GABA-A receptor (Zhu et al., 2018). Finally, Fab fragments derived from the antibody were used to facilitate crystallisation and improve stabilisation of the α1β2γ2 GABA-A receptor (Zhu et al., 2018).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: gamma-aminobutyric acid type A receptor alpha1 subunit
• Database Link:
  Entrez Gene 2554 Human
  UniProt ID P14867
• Synonyms: ECA4; EJM; EJM5