CD95 (FAS) Rabbit Polyclonal Antibody

CAT#: TA388940

sFas Receptor Antibody (biotin)

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: To detect Human sFas Receptor by sandwich ELISA (using 100 ml/well) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti- Human sFas Receptor as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human sFas Receptor.
  Western Blot
  To detect Human sFas Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Human sFas Receptor is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Human sFas Receptor. Anti-Human sFas Receptor specific antibody was purified by affinity chromatography and then biotinylated.
• Concentration: lot specific
• Purification: sFas Receptor specific antibody was purified by affinity chromatography and then biotinylated
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Fas cell surface death receptor
• Database Link:
  Entrez Gene 355 Human
  UniProt ID P25445
• Background: Fas and Fas Ligand (FasL) belong to the TNF superfamily, and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD, followed by processing of the pro-enzyme into active forms. These active caspases then cleave various cellular substrates, leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319 amino acid type I transmembrane protein, which contains a 157 amino acid extracellular domain, a 17 amino acid transmembrane domain, and a 145 amino acid cytoplasmic domain. Recombinant Human soluble Fas (sFas Receptor) is a 157 amino acid polypeptide (17.6 kDa) corresponding to the TNFR-homologous cysteine-rich extracellular Fas domain.
• Synonyms: ALPS1A; APO-1; APT1; CD95; FAIM2; FAS1; FASTM; LFG; NMP35; TNFRSF6