Amphiregulin (AREG) Rabbit Polyclonal Antibody

CAT#: TA388961

Amphiregulin Antibody

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: To detect Human Amphiregulin by sandwich ELISA (using 100ul/well), a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Amphiregulin as a detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human Amphiregulin.
  Western Blot
  To detect Human Amphiregulin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human Amphiregulin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits immunized with highly pure Recombinant Human Amphiregulin. Anti-Human Amphiregulin-specific antibody was purified by affinity chromatography employing an immobilized Human Amphiregulin matrix.
• Concentration: lot specific
• Purification: Amphiregulin-specific antibody was purified by affinity chromatography employing an immobilized Human Amphiregulin matrix
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: amphiregulin
• Database Link:
  Entrez Gene 374 Human
  UniProt ID P15514
• Background: Amphiregulin is an EGF related growth factor that signals through the EGF/TGF-alpha receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts. Amphiregulin also inhibits the growth of certain carcinoma cell lines. Synthesized as a transmembrane protein, Amphiregulin’s extracellular domain is proteolytically processed to release the mature protein. There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity. Recombinant Human Amphiregulin is an 11.3 kDa glycoprotein consisting of 98 amino acid residues.
• Synonyms: amphiregulin; AR; CRDGF; MGC13647; SDGF