ULK1 Rabbit Polyclonal Antibody

CAT#: TA392694

ULK1 (phospho-Ser555) polyclonal antibody

Product Images

Western blot (WB) analysis of PIK3C3/VPS34 (Phospho-Ser249) polyclonal antibody at 1:500 dilution Lane1:LO2 treated with PBS(1×,PH7.4) for 1 hour then treated with DMEM(10%FBS) for 15 minutes whole cell lysate Lane2:LO2 treated with PBS(1×,PH7.4) for 1 hour then treated with DMEM(10%FBS) for 5 minutes whole cell lysate Lane3:LO2 treated with PBS(1×,PH7.4) for 1 hour whole cell lysate Lane4:LO2 whole cell lysate

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human ULK1 around the phosphorylation site of Ser555.
• Specificity: ULK1 (phospho-Ser555) polyclonal antibody detects endogenous levels of ULK1 protein only when phosphorylated at Ser555.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 150 kDa
• Gene Name: unc-51 like autophagy activating kinase 1
• Database Link:
  Entrez Gene 8408 Human
  UniProt ID O75385
• Background: ULK1 and ULK2 (for UNC-51-like kinase) encode similar amino-terminal serine/threonine kinase domains, a proline/serine-rich (PS) domain, and a species conserved carboxyl-terminal domain. Both share homology with the UNC-51 kinase from Caenorhabditis elegans and the APG1 kinase in yeast, which are involved in axonal extension and growth, and autophagy, respectively. ULK1 and ULK2 are thought to auto-phosphorylate the PS domain in vitro, and the significant homology among vertebrates suggest that ULK1 and ULK2 are involved in the regulation of fundamental biological processes.
• Synonyms: ATG1; Autophagy-related protein 1 homolog; KIAA0722; Serine/threonine-protein kinase ULK1; ULK1; Unc-51-like kinase 1
• Note: For research use only, not for use in diagnostic procedure.