HES1 Mouse Monoclonal Antibody [Clone ID: OTI4H1]

CAT#: TA400011

Anti-HES1 mouse mAb, clone OTI4H1, DyLight488 conjugated

Product Images

HEK293T cells were transfected with pCMV6-ENTRY control (PS01, left panel) or HES1 cDNA (right panel) and incubated for 48 hours after transfection. The cells were detached, fixed with 2% formaldehyde for 30 min, permeabilized with 0.1% trixon-X100, and then stained with DyLight488-labeled HES1 antibodies (TA400011).

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  HEK293T cells were transfected with pCMV6-ENTRY control (PS01, left panel) or HES1 cDNA (right panel) and incubated for 48 hours after transfection. The cells were detached, fixed with 2% formaldehyde for 30 min, permeabilized with 0.1% trixon-X100, and then stained with DyLight488-labeled HES1 antibodies (TA400011).

Specifications

Product Data

• Clone Name: OTI4H1
• Applications: FC
• Recommended Dilution: FC: 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 1-280 of human HES1
• Formulation: PBS (pH7.4) containing 50% glycerol, 0.1% BSA and 0.02% NaN3
• Concentration: 0.5 mg/ml
• Purification: Purified from cell culture supernatant by affinity chromatography
• Conjugation: DyLight 488
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.4 kDa
• Gene Name: hes family bHLH transcription factor 1
• Database Link:
  NP_005515
  Entrez Gene 3280 Human
  UniProt ID Q14469
• Background: This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
• Synonyms: bHLHb39; HES-1; HHL; HRY

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young, Notch signaling pathway