Isocitrate dehydrogenase (IDH1) Mouse Monoclonal Antibody [Clone ID: OTI2H9]

CAT#: TA400028

Anti-IDH1 mouse mAb, clone OTI2H9, DyLight488 conjugated

Product Images

HEK293T cells were transfected with pCMV6-ENTRY control (PS01, left panel) or IDH1 cDNA (right panel) and incubated for 48 hours after transfection. The cells were detached, fixed with 2% formaldehyde for 30 min, permeabilized with 0.1% trixon-X100, and then stained with PE-labeled IDH1 antibodies (TA400028).

Specifications

Product Data

• Clone Name: OTI2H9
• Applications: FC
• Recommended Dilution: FC: 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human IDH1 expression vector
• Formulation: PBS (pH7.4) containing 50% glycerol, 0.1% BSA and 0.02% NaN3
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids by affinity chromatography
• Conjugation: DyLight 488
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.7 kDa
• Gene Name: isocitrate dehydrogenase (NADP(+)) 1
• Database Link:
  NP_005887
  Entrez Gene 3417 Human
  UniProt ID O75874
• Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
• Synonyms: HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD

Reference Data

• Protein Pathways: Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways