GATA6 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2B3]

CAT#: TA500186AM

GATA6 mouse monoclonal antibody, clone OTI2B3 (formerly 2B3), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA6 (RC220721, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA6. Positive lysates LY401617 (100ug) and LC401617 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-GATA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500186)

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  Anti-GATA6 mouse monoclonal antibody (TA500186) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GATA6 (RC220721).

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  Immunofluorescent staining of HT29 cells using anti-GATA6 mouse monoclonal antibody (TA500186).

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-GATA6 monoclonal antibody (1:200).

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Specifications

Product Data

• Clone Name: OTI2B3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:50, IF 1:50~100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 300-449 of human GATA6 (NP_005248) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 59.9 kDa
• Gene Name: GATA binding protein 6
• Database Link:
  NP_005248
  Entrez Gene 14465 MouseEntrez Gene 29300 RatEntrez Gene 2627 Human
• Background: The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells (Tsai et al., 1994 [PubMed 8078582]). GATA6 Thought to be important for regulating terminal differentiation and/or proliferation.
• Synonyms: GATA-binding protein 6; GATA binding protein 6; transcription factor GATA-6

Reference Data

• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors