BHMT Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3E11]

CAT#: TA500959BM

Anti-BHMT mouse monoclonal antibody, clone OTI3E11 (formerly 3E11), HRP conjugated

Product Images

HEK293T cells transfected with either pCMV6-ENTRY BHMT (RC203148) (Red) or empty vector control plasmid (Blue) were immunostained with anti-BHMT mouse monoclonal (TA500959), and then analyzed by flow cytometry.

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  Western blot analysis of extracts (10ug) from HepG2 cell line by using anti-BHMT monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BHMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500959, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-BHMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500959, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-BHMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500959, Dilution 1:50)

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-BHMT monoclonal antibody (1:200).

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Specifications

Product Data

• Clone Name: OTI3E11
• Applications: FC, IF, IHC, WB
• Recommended Dilution: IHC 1:200, WB: 1:200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BHMT (NP_001704) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45 kDa
• Gene Name: betaine--homocysteine S-methyltransferase
• Database Link:
  NP_001704
  Entrez Gene 12116 MouseEntrez Gene 81508 RatEntrez Gene 635 Human
  UniProt ID Q93088
• Background: This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.
• Synonyms: BHMT1; HEL-S-61p

Reference Data

• Protein Pathways: Cysteine and methionine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways