GRASP65 (GORASP1) Mouse Monoclonal Antibody [Clone ID: OTI4F8]

CAT#: TA501392S

Anti-GORASP1 (GRASP65) mouse monoclonal antibody, clone OTI4F8 (formerly 4F8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GORASP1 (RC210076, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GORASP1. Positive lysates LY403127 (100ug) and LC403127 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-GORASP1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Anti-GORASP1 mouse monoclonal antibody (TA501392) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GORASP1 (RC210076).

Specifications

Product Data

• Clone Name: OTI4F8
• Applications: IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GORASP1 (NP_114105) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.98 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.3 kDa
• Gene Name: golgi reassembly stacking protein 1
• Database Link:
  NP_114105
  Entrez Gene 64689 Human
  UniProt ID Q9BQQ3
• Background: The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined. [provided by RefSeq]
• Synonyms: GOLPH5; GRASP65; P65