NEK11 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI5E9]

CAT#: TA501608BM

Anti-NEK11 mouse monoclonal antibody, clone OTI5E9 (formerly 5E9), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK11 (RC221953, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK11. Positive lysates LY411057 (100ug) and LC411057 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC221953 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NEK11 antibody (TA501608), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI5E9
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NEK11 (NP_079076) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74.0 kDa
• Gene Name: NIMA related kinase 11
• Database Link:
  NP_079076
  Entrez Gene 79858 Human
  UniProt ID Q8NG66
• Background: This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.
• Synonyms: FLJ23495

Reference Data

• Protein Families: Druggable Genome, Protein Kinase