Growth Arrest Specific Protein 7 (GAS7) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2F6]

CAT#: TA501756AM

Anti-GAS7 (Growth Arrest Specific Protein 7) mouse monoclonal antibody, clone OTI2F6 (formerly 2F6), Biotinylated

Product Images

HEK293T cells transfected with either RC215256 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GAS7 antibody (TA501756), and then analyzed by flow cytometry.

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  Anti-GAS7 mouse monoclonal antibody (TA501756) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GAS7 (RC215256).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GAS7 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GAS7 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAS7 (RC215256, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAS7. Positive lysates LY418527 (100ug) and LC418527 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GAS7 monoclonal antibody.

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Specifications

Product Data

• Clone Name: OTI2F6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog, Rat, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GAS7 (NP_003635) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.0 kDa
• Gene Name: growth arrest specific 7
• Database Link:
  NP_003635
  Entrez Gene 14457 MouseEntrez Gene 85246 RatEntrez Gene 608189 DogEntrez Gene 8522 Human
  UniProt ID O60861
• Background: Growth arrest-specific 7 is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development. Several transcript variants encoding proteins which vary in the N-terminus have been described. [provided by RefSeq]
• Synonyms: GAS7; MLL

Reference Data

• Protein Families: Transcription Factors