HSPA6 Mouse Monoclonal Antibody [Clone ID: OTI1H3]

CAT#: TA501773

HSPA6 mouse monoclonal antibody, clone OTI1H3 (formerly 1H3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA6 (RC207795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA6. Positive lysates LY419498 (100ug) and LC419498 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA6 monoclonal antibody.

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  Anti-HSPA6 mouse monoclonal antibody (TA501773) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA6 (RC207795).

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  HEK293T cells transfected with either RC207795 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HSPA6 antibody (TA501773), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1H3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HSPA6 (NP_002146) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70.8 kDa
• Gene Name: heat shock protein family A (Hsp70) member 6
• Database Link:
  NP_002146
  Entrez Gene 3310 Human
  UniProt ID P17066
• Synonyms: heat shock 70kDa protein 6 (HSP70B'); heat shock 70kD protein 6 (HSP70B')

Reference Data

• Protein Pathways: Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Spliceosome