Doublecortin (DCX) Mouse Monoclonal Antibody [Clone ID: OTI4A3]

CAT#: TA501778S

DCX mouse monoclonal antibody, clone OTI4A3 (formerly 4A3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCX (RC221891, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCX. Positive lysates LY403598 (100ug) and LC403598 (20ug) can be purchased separately from OriGene.

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  Immunofluorescent staining of 293T cells transiently transfected by pCMV6-ENTRY DCX (RC221891) using anti-DCX mouse monoclonal antibody (TA501778, green, upper left; merged, upper right). Cell nuclei were stained with DAPI (blue, lower left). 293T cells transfected with empty vector plasmid served as negative control ( merged, lower right) (1:100).

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  Flow cytometric Analysis of Jurkat cells, using anti-DCX antibody (TA501778), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Western blot analysis of extracts (35ug) from 2 tissues by using anti-DCX monoclonal antibody (1:250).

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  Living HEK293T cells transfected with either RC221891 plasmid (red) or empty vector (blue) were immunostained by anti-DCX antibody (TA501778) or isotype control antibody (green), and then analyzed by flow cytometry (1:100).

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Specifications

Product Data

• Clone Name: OTI4A3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DCX (NP_835365) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.73 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.4 kDa
• Gene Name: doublecortin
• Database Link:
  NP_835365
  Entrez Gene 13193 MouseEntrez Gene 84394 RatEntrez Gene 1641 Human
  UniProt ID O43602
• Background: This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ('double cortex' syndrome) in females and lissencephaly ('smooth brain' syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: DBCN; DC; LISX; SCLH; XLIS

Reference Data

• Protein Families: Druggable Genome