MRPS2 Mouse Monoclonal Antibody [Clone ID: OTI4D6]

CAT#: TA502031S

MRPS2 mouse monoclonal antibody, clone OTI4D6 (formerly 4D6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS2 (RC203579, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS2. Positive lysates LY414231 (100ug) and LC414231 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MRPS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502031)

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  HEK293T cells transfected with either RC203579 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MRPS2 antibody (TA502031), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-MRPS2 antibody (TA502031), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-MRPS2 antibody (TA502031), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI4D6
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MRPS2 (NP_057118) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1.2 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.1 kDa
• Gene Name: mitochondrial ribosomal protein S2
• Database Link:
  NP_057118
  Entrez Gene 51116 Human
  UniProt ID Q9Y399
• Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S2 family. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: CGI-91; MRP-S2; S2mt