PYCR2 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI5C7]

CAT#: TA502144BM

PYCR2 mouse monoclonal antibody, clone OTI5C7 (formerly 5C7), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCR2 (RC204525, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCR2. Positive lysates LY402246 (100ug) and LC402246 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PYCR2 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PYCR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502144)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PYCR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502144)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PYCR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502144)

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  Anti-PYCR2 mouse monoclonal antibody (TA502144) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2 (RC204525).

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Specifications

Product Data

• Clone Name: OTI5C7
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PYCR2 (NP_037460) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.5 kDa
• Gene Name: pyrroline-5-carboxylate reductase 2
• Database Link:
  NP_037460
  Entrez Gene 69051 MouseEntrez Gene 364064 RatEntrez Gene 29920 Human
  UniProt ID Q96C36
• Synonyms: HLD10; P5CR2

Reference Data

• Protein Pathways: Arginine and proline metabolism, Metabolic pathways