BIN3 Mouse Monoclonal Antibody [Clone ID: OTI8E9]

CAT#: TA502145

BIN3 mouse monoclonal antibody, clone OTI8E9 (formerly 8E9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIN3 (RC203378, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIN3. Positive lysates LY402705 (100ug) and LC402705 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BIN3 monoclonal antibody.

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-BIN3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502145)

• 

  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-BIN3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502145)

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BIN3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502145)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BIN3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502145)

• Anti-BIN3 mouse monoclonal antibody (TA502145) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BIN3 (RC203378).

• HEK293T cells transfected with either RC203378 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BIN3 antibody (TA502145), and then analyzed by flow cytometry.

Show all 8 images

Specifications

Product Data

• Clone Name: OTI8E9
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BIN3 (NP_061158) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.69 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.5 kDa
• Gene Name: bridging integrator 3
• Database Link:
  NP_061158
  Entrez Gene 57784 MouseEntrez Gene 361065 RatEntrez Gene 608969 DogEntrez Gene 708840 MonkeyEntrez Gene 55909 Human
  UniProt ID Q9NQY0
• Background: The product of this gene is a member of the BAR domain protein family. The encoded protein is comprised solely of a BAR domain which is predicted to form coiled-coil structures and proposed to mediate dimerization, sense and induce membrane curvature, and bind small GTPases. BAR domain proteins have been implicated in endocytosis, intracellular transport, and a diverse set of other processes. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: MGC14978